Protective effects of Shen-fu injection on hemodynamic parameters and lung injury in a porcine model of hemorrhagic shock
Qiang Zhang, Zhao Liu, Gu Wei, Bo LiuBackground:
Since it remains unclear whether Shen-fu injection (SFI) could be used in hemorrhagic shock (HS), this study aimed to explore the effects of SFI on lung injury caused by HS in a porcine model.
Methods:
After HS induction, we randomly assigned 32 pigs into 4 groups: group I (n = 8, no resuscitation); group Ⅱ (n = 8, resuscitated with shed blood); group III (n = 8, resuscitated with shed blood and saline at a dose of 3 mL/kg); and group IV (n = 8, resuscitated with shed blood and SFI at a dose of 3 mL/kg. After 6 hours of HS, we analyzed interleukin (IL)-6, IL-8, IL-4, and IL-10 levels of lung tissue using an enzyme-linked immunosorbent assay. Apoptosis in the lung was evaluated using an immunohistochemistry staining assay and transmission electron microscopy.
Results:
In a porcine model of HS, 23 of 32 animals survived for 6 hours across 4 groups. Cytokine analysis revealed that group IV exhibited significantly reduced levels of proinflammatory cytokines, including IL-6 and IL-8, concomitant with elevated concentrations of anti-inflammatory cytokines, specifically IL-4 and IL-10 (
Conclusion:
SFI may protect against lung injury in a porcine HS model.