DOI: 10.62425/jlasp.1977803 ISSN: 2791-8645

Protective Effect of Active Anionic Oxygen Administration on Testicular Tissue in Rats with Experimental Sepsis

Ömer Faruk Başer, Mahmut Karapehlivan
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This study aimed to investigate the effects of active anionic oxygen (AAO) on oxidative stress, inflammation, and steroidogenesis in sepsis-induced testicular injury in rats. Thirty male Wistar Albino rats were randomly assigned to four groups: Control, Sepsis (lipopolysaccharide, LPS), AAO, and Sepsis+AAO. Testicular tissues were analyzed for oxidative stress markers (reduced glutathione [GSH], malondialdehyde [MDA], superoxide dismutase [SOD], and catalase [CAT]), inflammatory cytokines (interleukin-6 [IL-6], tumor necrosis factor-alpha [TNF-α], transforming growth factor-beta 1 [TGF-β1], and interleukin-1 beta [IL-1β]), and steroidogenic markers (3β-hydroxysteroid dehydrogenase [3β-HSD], 17β-hydroxysteroid dehydrogenase [17β-HSD], and steroidogenic acute regulatory protein [StAR]). Sepsis significantly decreased GSH (p < .0001), SOD (p < .01), and CAT (p < .0001) levels, while increasing MDA (p < .0001) levels compared with the control group. In addition, IL-6 (p < .0001), TNF-α (p < .001), TGF-β1 (p < .05), and IL-1β (p < .0001) levels were significantly elevated, whereas the expression of 3β-HSD (p < .01), 17β-HSD (p < .05), and StAR (p < .01) was significantly reduced. Compared with the sepsis group, AAO treatment significantly improved antioxidant status (all p < .05), suppressed inflammatory cytokine levels (all p < .01), and enhanced steroidogenic marker expression (all p < .05). These findings demonstrate that AAO exerts potent antioxidant and anti-inflammatory effects, attenuates sepsis-induced testicular injury, and preserves steroidogenesis, suggesting its potential as a protective agent for sepsis-associated testicular dysfunction.

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