Promising antidiabetic potential of traditionally used plant Coccinia indica in streptozotocin-induced diabetic rat model
Anisur Rahman, Asif Ahmad Khan, Sarfaraz Ahmad, Mohammed Abdul Qadeer, Shabnam, Saima NaseemObjectives
Diabetes continues to affect millions of people globally despite the existence of conventional antidiabetic medications. The objective of this research was to assess the anti-diabetic effects of Coccinia indica root extract (CIE) in rats that had diabetes induced by streptozotocin (STZ).
Material and Methods
Coccinia indica root extract was prepared by alcoholic extraction and characterized for phytochemical content. In vitro antidiabetic potential was evaluated through alpha-amylase inhibition and antioxidant assays using HepG2 cells. In vivo efficacy was assessed in streptozotocin-induced diabetic rats via blood glucose measurement, serum biochemical analysis, and histopathological examination. Statistical analysis was performed on all data across control, diabetic, and treatment groups.
Results
The results indicated that CIE demonstrated potent inhibitory actions against alpha-amylase while displaying favorable compatibility with HepG2 cells. Daily CIE treatment for 21 days normalized the blood glucose levels and tumor necrosis factor-alpha (TNF-α) expression in diabetic control rats. Furthermore, it improved liver and kidney function and mitigated dyslipidemia and liver antioxidant enzyme abnormalities. Histological analysis of the treated group revealed reduced damage to the pancreatic, hepatic, and renal tissues, corroborating CIE’s antidiabetic efficacy.
Conclusion
These findings suggest that CIE is a potential therapeutic option for diabetes management.