DOI: 10.5958/0974-0171.2025.00004.7 ISSN: 0971-4251
Potential of MDCK and A-72 cell lines in isolation and adaption of canine parvovirus-2 (CPV-2)
Rajendran Manikandan, Amritha S Kumar, Gore Venu, Anuradha B Bhandiwad, Arpita Sain, R. Saravanan, Vishal Chander, Gaurav Kumar Sharma, Ujjawal K. De, Mithilesh SinghThe paper reports screening of clinical faecal samples (n=10) and 4 samples were found positive by PCR and adapted in MDCK and A72 cell lines. The virus was propagated for three passages, in which A72 cell line displayed more prominent cytopathic effect (CPE) as compared to MDCK cells. In correspondence, positive agglutination with HA titer was 512 in A72 and 128 in MDCK infected cells. The average virus titre was higher in A72 with 105.89 TCID50/mlcompared to MDCK of 104.77 TCID50/ml.