DOI: 10.17826/cumj.1798037 ISSN: 2602-3032

Potential effects of Amygdalin on cytotoxicity, apoptosis, oxidative damage, and cell cycle related genes expressions in H1650 lung adenocarcinoma cells

Gonca Gülbay, Mücahit Seçme, Sümeyya Deniz Aybek
Show PDF Cite
Purpose: The aim of this study is to determine the anticancer effects of the active ingredient amygdalin on H1650 lung cancer cells.Materials and Methods: The antiproliferative effect of amygdalin on H1650 lung cancer cells was evaluated using the MTT assay. Its effect on oxidative damage was determined using 8-OHdG and total antioxidant status (TAS), total oxidant status (TOS), and by calculating the oxidative stress index (OSI). Its effect on apoptosis was demonstrated using the caspase-3 ELISA method. Changes in mRNA expression of the CDK1, CDK2, CDK4, CDK6, Cyclin D1, Cyclin B1, and Cyclin E genes were assessed by RT-PCR.Results: The IC50 dose of amygdalin in H1650 cells was determined to be 443.8 μM at 24 h. A significant decrease in the expression of CDK1 (0.48-fold), CDK2 (0.31-fold,), CDK6 (0.16-fold), Cyclin D1 (0.24-fold,), Cyclin B1 (0.07-fold), and Cyclin E (0.05-fold) genes was observed in the group treated with amygdalin. The differences in TOS, TAS and OSI levels and in 8-OHdG and caspase-3 levels between groups were not statistically significant. Conclusion: Amygdalin shows potential as an anticancer agent and may contribute to the development of different therapeutic approaches for lung cancers.

More from our Archive