Phytochemical investigation and effects of Artemisia santolinifolia extract on biochemical parameters in diabetic animal model

Abstract

In this piece of work, the phytochemical profile of fruit extract of Artemisia santolinifolia was investigated using HPLC and GC-MS analysis. The extract was then subjected to animal’s rat model for its effect on biochemical and hematological parameters in diabetic animals. The GC-MS and HPLC analysis reflect presence of known chemical compounds. The plant extract lowered the blood glucose level to 191.54 and 177.39 mg/dL at test doses of 125 and 250 mg/kg body weight when compared to diabetic animals having blood glucose level of 416.21 ± 4.20 mg/dL. Similarly, the extracts at doses of 125 and 250 mg/kg lowered the cholesterol level to 97.21 and 110.97 mg/dL correspondingly when compared to diabetic animals (217.25 ± 2.14). The fruit extract reversed the biochemical parameters toward normal range. Diabetes influenced the WBC count in the diabetic group, which was reversed by plant extract. A moderate change in RBC level for the diabetic group was also noted. PCV% and Hb levels were also moderately affected by induced diabetes, which were effectively reversed by plant extract. In light of the current study it may be concluded that the extract is rich in phytochemicals which are biologically active and has the ability to reverse the changes that were caused or produced by diabetes.