Photoinduced Chemiluminescence Determination of Prednisolone Using Lucigenin‐Cobalt 5,10,15,20‐Tetra(4‐Pyridyl)‐21H,23H‐Porphine System

ABSTRACT

Sensitive and precise assay of prednisolone, a steroid hormone, has received great attention due to its significant role in the treatment of a series of diseases. In this study, we have developed a simple, quick, and accurate technique in this part to measure prednisolone. Cobalt 5,10,15,20‐tetra(4‐pyridyl)‐21H,23H‐porphine (CoTPyP) as a mimic peroxidase catalyzes the chemiluminescence (CL) reaction of lucigenin for the first time. CoTPyP has a great impact on the CL signal. Furthermore, the CL system has been successfully used for the prednisolone flow‐injection test. The findings showed that the UV irradiation of prednisolone led to an unimolecular photochemical reaction that could enhance yield of high‐energy intermediate and cause higher CL emission. Under ideal experimental circumstances, CL intensity was shown to be proportionate to prednisolone concentration in the range of 0.1–170 μg mL⁻¹ (r = 0.9925, n = 4). A detection limit of 0.072 μg mL⁻¹ was observed. Compared with the HPLC approach, our proposed method provides a sensitive, fast, and efficient analysis of prednisolone in pharmaceutical formulations and biological fluids.