Phenol‐Grafted Polysaccharide Hydrogels Activated By Humid Air Plasma for Controlled Reactive Oxygen and Nitrogen Species (RONS) Delivery
Souwaidi Ali, Rohan Quoniou, Imen Ghzaiel, Jean‐Marc Lobaccaro, Silvère Baron, Emmanuel Petit, Redouan El Boutachfaiti, Fréderic Perisse, Cédric Delattre, Sébastien MenecierABSTRACT
Cold atmospheric plasma enables the generation of reactive oxygen and nitrogen species (RONS) for biomedical use. This work develops plasma activated polysaccharide hydrogels by exposing phenol functionalized alginate, PGU, and xanthan to humid air plasma, which boosts H 2 O 2 production and triggers HRP‐mediated gelation without added peroxide. The resulting materials show pseudoplastic, self‐healing behavior, with mechanics tuned by polymer composition. Humid plasma increases incorporation of H 2 O 2 , NO 2 − , and NO 3 − , the latter being dominant. Xan Ph releases the most RONS, while PGU Ph retains less due to HRP consumption during polymerization. These findings demonstrate that humid plasma can simultaneously polymerize hydrogels and load them with RONS, offering a platform for injectable plasma activated biomaterials suited for cancer therapy, tissue engineering, and 3D bioprinting.