Pharmacological Effects of Rosa damascena Mill. ‘Hetian’ on Type 2 Diabetes Mellitus With Hyperlipidemia
Yanrui Chen, Liting Ruan, Min Wang, Xiaoxi Zhang, Mengsha Li, Chaohui Zeng, Jianyu Zhang, Zhen Xiang, Xu BaiObjectives
Type 2 diabetes mellitus\T2DM with hyperlipidemia is a major risk factor for accelerating atherosclerosis and cardiovascular diseases, with a complex etiology and limited treatment options. Although the flowers of
Methods
An integrated strategy combining network pharmacology, molecular docking, molecular dynamics (MD) simulations, and in vivo validation in a T2DM mouse model was employed. Network pharmacology predicted potential targets and bioactive compounds. Key pathways were enriched via GO and KEGG analyses. Molecular docking and 100 ns MD simulations using GROMACS evaluated binding stability. In vivo experiments assessed fasting blood glucose, glucose tolerance, insulin sensitivity, and serum lipid profiles in T2DM mice after the ultra-micro powder of
Results
Network pharmacology identified 143 potential targets and quercetin, vitamin E, demethoxycapillarisin, β-sitosterol, and sitosterol as top bioactive compounds. Key enriched pathways included insulin resistance and PI3K-Akt. Molecular docking and 100 ns MD simulations confirmed stable binding of quercetin to PTGS2. In vivo experiments demonstrated that RUP treatment (1.0, 2.0, 3.0 g/kg) significantly lowered fasting blood glucose, improved glucose tolerance and insulin sensitivity, and ameliorated serum lipid profiles (TG, TC, LDL-C, HDL-C) in T2DM mice.
Conclusion