Pathogen-Induced Changes in Lipopeptide Production from the Marine-Derived Streptomyces albidoflavus PNM-87 against Pyricularia oryzae
Vanesa Diaz-Rios, Jeimmy Macias Camacho, Lina M. Bayona Maldonado, Diana Vinchira-Villaraga, Olga Higuera Acosta, Nubia Moreno Sarmiento, Leonardo Castellanos Hernandez, Freddy A. RamosAbstract
The emergence of fungicide resistance in Pyricularia oryzae necessitates searching for efficient biocontrol strains from under-explored environments. This study reports P. oryzae control from the marine sponge-associated Streptomyces albidoflavus PNM-87. Initial screenings revealed that PNM-87 exhibits significant in vitro inhibition against rice blast. Active compounds were characterized by Feature-Based Molecular Networking (FBMN) and SIRIUS as a mixture of surfactin-like lipopeptides including surfactins (BC14, C13, C15) and pumilacidins (B, D/E), representing 82% of the total ion current (TIC). Comparative metabolic profiling in pathogen-supplemented media revealed pathogen-induced changes in the specialized metabolome; PNM-87 upregulated surfactin and pumilacidin B production by up to 200- and 400-fold, respectively. Greenhouse assays confirmed plant protection, reducing rice blast severity in O. sativa plants. This work provides the first evidence of a marine-derived S. albidoflavus strain employing a targeted chemical defence strategy for agricultural pathogen suppression, highlighting the potential of marine actinobacteria as biocontrol agents.