P1325 Metabolic dynamics of the small intestinal microbiota upon nutritional interventions
S Jordi, J Li, I Bärtschi, N Fasel, W Jacqueline, B Stecher, U Sauer, M Andrew, B Yilmaz, B MisselwitzAbstract
Background
The human small intestinal microbiota, which plays a crucial role in intestinal function, remains poorly investigated due to limited accessibility.
Methods
We utilise the access via Ileostomies in a randomised cross-over trial with two nutritional interventions (high carb vs. high fat). Stoma effluent samples are collected from 70 stoma patients hourly (0-6h) after the intervention, along with blood and urine samples (0h, 3h, 6h). Stoma effluent samples from each study day are anaerobically cultured to generate a collection of small intestinal bacteria strains.
Results
We characterise the small intestinal microbiota through taxonomy (16S sequencing), biomass, and metabolome analysis. Shotgun metagenomic and mRNA sequencing are performed at selected timepoints. Our comprehensive “multi-omics" analysis involves identifying bacterial networks, interpreting their dynamics. In parallel, we have created a bacterial isolate catalogue (>1000 isolates, ~200 species) form human samples to reproduce key interactions in vitro.
Conclusion
This study aims for a comprehensive analysis of small intestinal microbiota dynamics in the context of different nutritional interventions. The data and samples collected will enable hypothesis testing in vitro and lay the groundwork for future disease-specific investigations. Additionally, by establishing an extensive catalogue of well-characterised human-derived bacterial isolates, we created a resource for future experimental research projects.