P-105: Genes associated with diabetic nephropathy in adolescents with type 1 diabetes in serbia
M Jesic, S Kovacevic, V Zdravkovic, V Bojic, J BlagojevicAbstract
Introduction
Genetic studies may provide valuable information about patients who are at high risk of developing diabetic nephropathy. Before the onset of albuminuria, genetic mutations can predispose to the development of kidney disease.
Objectives
This study aimed to examine clinical and laboratory differences between adolescents with type 1 diabetes (T1D) who had and did not have microalbuminuria. Secondly, we examined the distribution of the ACE, AGTR1, and MTHFR gene polymorphisms in patients with T1D, divided into two groups depending on the presence or absence of microalbuminuria (MA).
Methods
The study included 130 adolescents with T1D. Patients were divided into two groups according to the presence of microalbuminuria. This study was performed to examine clinical and laboratory differences between adolescents with T1D with and without MA and the distribution of the ACE, AGTR1, and MTHFR gene polymorphisms.
Results
The mean MA in the first group, 6.41±7.35, significantly differs from the second group, 0.82±0.48 (p<0.001). HbA1c, 24-hour proteinuria, and daytime systolic blood pressure were significantly higher in the MA group (p<0.05). A smaller systolic blood pressure percentage nocturnal decline was observed in the MA group (p 0.030). The frequencies of the ACE DD, ID, and II genotypes were 12.5%, 50.0%, and 37.5%, respectively, among T1D patients with MA, and 19.3%, 56.1%, 24.6%, in the control group without MA (p 0.510). The frequencies of the AGTR1 AA, AC, and CC genotypes were 62.5%, 25.0%, and 12.5% among TID patients with MA, and 49.1%, 43.9%, 8.0%, in the group without MA (p 0.326). The frequencies of the MTHFR CC, CT, and TT genotypes were 37.5%, 50.0%, 12.5% among TID patients with MA, and 37.7%, 45.6%, 16.7% in the group without MA (p 0.901).
Conclusions
Our data suggest that common variants in the AGTR1, ACE, and MTHFR genes are not strongly associated with diabetic nephropathy in our patients with T1D.