On the possibility of determining the species of saliva stains using quantitative enzyme immunoassay

Objective. Determination of the species identity of saliva samples dried on gauze based on total human IgG using quantitative enzyme immunoassay. Material and methods. Saliva samples taken from 10 donors and dried on gauze. The samples were tested by quantitative ELISA using the IgG-total-ELISA-HEMA test kit. Results. The optical density and concentration of total IgG in whole saliva extracts were determined in 1:10 and 1:50 dilutions. The sensitivity limit of the method was established. Conclusion. The experimental data obtained indicate a reliable identification of the origin of the studied saliva samples on gauze from humans, both in undiluted extracts and in a 1:10 dilution. This variant of quantitative ELISA has absolute specificity, sufficient sensitivity and high productivity, which determines the prospect of its use to establish the species identity of saliva traces.