DOI: 10.1099/ijsem.0.006828 ISSN: 1466-5026
Oceaniferula spumae sp. nov., a novel Verrucomicrobiota bacterium, isolated from sea foam at Noto Peninsula, Ishikawa, Japan
Can Huang, Chunqi Jiang, Yuki Sato-Takabe, Yoko Makabe-Kobayashi, Yuya Tsukamoto, Susumu Yoshizawa, Koji HamasakiA Gram-stain-negative, aerobic, rod-shaped bacterium, designated as NT6NT, was isolated from sea foam collected at the Maura Coast of the Noto Peninsula, Ishikawa, Japan. Strain NT6NT was able to grow optimally at pH 7.8, 25–30 °C with 3.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NT6NT belonged to the genus Oceaniferula of the family Verrucomicrobiaceae and was most closely related to Oceaniferula flava corrig. 5K15T (98.3%) and Oceaniferula marina N1E253T (95.7%). Furthermore, in silico average
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identity values between strain NT6NT and its closest related species, O. flava 5K15T and O. marina N1E253T, were 76.2% and 70.5%, respectively. The digital DNA–DNA hybridization values for the two comparisons were 20.2% and 19.6%, respectively. The chemotaxonomic characteristics showed that strain NT6NT had MK-9 and MK-8 as its main components of menaquinone and the main cellular fatty acids were iso-C14 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C12 : 0. The major polar lipids of strain NT6NT comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The genome of strain NT6NT had a length of 4.65 Mbp with 52.1 mol% G+C content, containing 3,914 protein-coding genes and 68 RNA genes. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain NT6NT represents a novel species within the genus Oceaniferula, for which the name Oceaniferula spumae sp. nov. is proposed. The type strain is NT6NT (=JCM 36658T=DSM 117338T=MCCC 1K09726T).