DOI: 10.1039/d6ra05175h ISSN: 2046-2069
Novel glutaminase-free
l
-asparaginase from
Leuconostoc mesenteroides
BN1: biochemical properties, molecular dynamics, and anticancer activity
Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Yanisa Srichompoo, Praphasri Septham, Phatchanat Klaihmon, Sujittra Khampang, Surapol Issaragrisil, Chanchao Lorthongpanich Leuconostoc mesenteroides
l
-asparaginase (
Lm
ASNase) exhibits high specificity for
l
-asparagine with no detectable
l
-glutaminase activity. MD simulations support selective
l
-asparagine binding, and
Lm
ASNase exhibits anticancer activity.