Nocardioides pymetrozini sp. nov., a pymetrozine-degrading bacterium isolated from long-term continuous cropping cotton fields

A novel bacterial strain, designated as R1-1 ^T , was isolated from the saline-alkaline soil of a cotton field in Xinjiang, China. Strain R1-1 ^T grew at 20–45 ℃, pH 4.0–11.0 and 0–10% NaCl. Notably, this strain degraded 50 mg l ⁻¹ pymetrozine within 18 h at pH 8.0. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain R1-1 ^T belongs to the genus Nocardioides , showing a high sequence similarity to ‘ Nocardioides carbamazepini ’ CBZ-1 ^T (98.5%). Other closely related species included Nocardioides albidus THG-S11.7 ^T (98.0%), Nocardioides convexus W3-2-3 ^T (98.0%), Nocardioides kongjuensis A2-4 ^T (98.0%) and Nocardioides daeguensis JCM 17460 ^T (97.4%). The genome sequence of strain R1-1 ^T had a G+C content of 72.2 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain R1-1 ^T and its closest phylogenetic relatives were 84.5–84.9% and 28.5–28.7%, respectively. These values were below the determined thresholds for species delineation. The major fatty acids of strain R1-1 ^T were identified as 10-methyl C _18 : 0 , iso-C _16 : 0 and C _18 : 1  ω9c. The predominant respiratory quinone was menaquinone-8 (H ₄ ), and the polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and two unidentified lipids. Based on the polyphasic taxonomic data, strain R1-1 ^T represents a novel species within the genus Nocardioides , for which the name Nocardioides pymetrozini sp. nov. is proposed. The type strain is R1-1 ^T (=KCTC 59241 ^T =GDMCC 4.365 ^T ).