DOI: 10.4103/jdras.jdras_87_24 ISSN: 2279-0357
Mountainous therapeutic plant Nardostachys jatamansi (D.Don) DC grown in vitro used as a substitute for wild plants
Hem Chandra Pant, Naveen Gaurav, Harsh Vardhan Pant Abstract
BACKGROUND:
Nardostachys jatamansi is widely used in the Unani, Ayurveda, and Siddha medicine systems. The extensive use of this plant in traditional medicine makes it an endemic plant. N. Jatamansi relieves symptoms of anxiety, epilepsy and weak memory, whereas plant oil (basically known as spikenard oil) is used to maintain hair growth, cardiac problems, and diabetes.
METHODS:
In our study, we tried to validate in vitro-grown plants as a substitute for wild-type plants for therapeutic purposes. We compared the phytochemical and biological (antibacterial) activity of in vitro-grown and wild-type plant root extract. For comparative analysis, we cultivated in vitro plants from the nodal ex-plants of N. Jatamansi. Then, methanol and water extract from in vitro grown and wild-type plant rhizome extract were prepared using the maceration method.
RESULTS:
The results revealed that in comparison to wild-type plants, other than flavonoids and terpenoids. All phytochemicals were reported in the in vitro-grown plant extract. However, both plant extracts showed a significant (P > 0.05) antibacterial activity.
CONCLUSION:
This study found that in vitro generated and wild-type plant extracts showed almost similar phytochemical profiles. Antimicrobial activity was also detected in plants grown in vitro. Our findings are suggestive of exploring utility of in vitro-grown plants as a substitute for wild type plants by investigating their phytochemical and pharmacological profile.