DOI: 10.4103/mjbl.mjbl_368_24 ISSN: 1812-156X

Molecular Genetic Determination AAC(6’)-Ib Gene Encoding AAC(6’)-I Enzyme among Aminoglycoside Resistant in Proteus Mirabilis Isolated from Clinical Samples

Noor Mohammed Zwaid, Ahmed Ali Mhawesh
Show PDF Cite

Abstract

Background:

Proteus mirabilis ( P. mirabilis ) is a common enterobacterial species that colonizes the gastrointestinal lumen and can cause severe infections in various human body systems. This opportunistic pathogen poses significant clinical challenges due to its production of aminoglycoside-modifying enzymes, which compromise the efficacy of aminoglycoside antibiotics and complicate infection management. The AAC(6’)-Ib gene is one of the most prevalent genes that determines the enzyme.

Objectives:

This study aimed to determine the prevalence of the AAC(6’)-Ib gene, which encodes for the AAC(6’)-I enzyme, among clinical isolates of P. mirabilis .

Materials and Methods:

Specimens from urine, wounds, and ear swabs were obtained in the clinical setting. After phenotypic and genotypic characterizations, the results showed the isolate to P. mirabilis . The susceptibility profile against 16 antibiotic classes was done, with clinical particularities to aminoglycoside antibiotics. PCR amplification detects AAC(6’)-Ib gene, and then sample sequencing and alignment were done.

Results:

A total of 200 samples were collected, including 125 (62.5%) from urine, 50 (25%) from wounds, and 25 (12.5%) from ear swabs. P. mirabilis was the most common organism detected in 50 (25%) of them, and extensive drug resistance was detected in 10 (20%) wherein many drugs proved useless. The most prominent resistance was shown to be the gentamicin. The AAC(6’)-Ib gene that was found harbored in all isolates (100%) was shown to be associated with resistance.

Conclusion:

The widespread prevalence of the AAC(6’)-Ib gene in extensively drug-resistant P. mirabilis isolates seriously threatens strategies for treatment. Regarding antibiotic effectiveness against P. mirabilis , ertapenem displayed the highest sensitivity among the tested antibiotics.

More from our Archive