DOI: 10.4103/mjbl.mjbl_622_23 ISSN: 1812-156X

Molecular Detection of Some Genes in Escherichia coli Isolated From Patient with Renal Failure

Khawla Abbas Alshemmari, Ali Abdul Kadhim Al-Ghanimi
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Abstract

Background:

Renal failure, both acute and chronic, is considered one of the health problems that threaten life and public safety, and causes death and many problems and complications that impede life performance and affect the psychological and social compatibility of the individual.

Objective:

The aim of this study was to determine the virulence factor in Escherichia coli .

Materials and Methods:

In this study, 160 urine samples were collected from patients with kidney failure and urinary tract infection (UTI) from different age groups ranging from (20‐70) years and for both sexes from patients who visited Imam Hussein Medical City.

Results:

22 (38.5%) E. coli out of a total of 57 samples were collected and 35 (61.4%) belonged to other bacterial genera in urine samples. After conducting molecular examination of these isolates using the polymerase chain reaction (PCR) technique, the number of isolates of these bacteria was confirmed and by using the same technique, many genes encoding virulence factors that make them capable of causing many diseases have been detected PCR.

Conclusion:

E. coli was the most frequently isolated kind of bacteria among those that cause renal failure and UTIs, and it is believed that the existence of the virulence factor determines the pathogenic potential of E. coli strains.

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