DOI: 10.4103/mjbl.mjbl_746_23 ISSN: 1812-156X

Molecular Detection of Multidrug Resistance Genes in Klebsiella Pneumoniae Isolates from Different Sources in Karbala Province

Nibras Saleh Ghafel Altememe, Kawkab A. H. Alsaadi
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Abstract

Background:

Metallo-β-lactamases (MBLs) are enzymes that certain bacteria produce to resist the effects of β-lactam antibiotics. They require zinc ions and can quickly degrade β-lactam antibiotics, making them ineffective. Metallo-lactamases of molecular class B (MBLs) [e.g., Verona integron-encoded metallo-β-lactamases (VIMs), imipenemase metallo-β-lactamases (IMPs), and New Delhi metallo-β-lactamases (NDMs)] are among the most typical carbapenemases.

Objective:

The study aims to detect blaIMP , blaVIM , and blaNDM genes from Klebsiella pneumonia which are isolated from different clinical samples in Kerbala Province.

Materials and Methods:

This study is cross-sectional 68 K. pneumoniae isolates were collected from patients with different infections, and isolates were identified by Vitek 2 automated system. Antibiotic susceptibility was measured using the disc diffusion method, and finally, the blaIMP , blaVIM , and blaNDM genes were detected using the polymerase chain reaction.

Results:

All isolates of K. pneumoniae used in this study were based on susceptibility testing with 12 antimicrobial (according to CLSI 2022 instructions). K. pneumoniae isolates were highly resistant to MBLs (e.g., VIMs, IMPs, NDMs) and are among the most typical carbapenemases sulfonamide.

Conclusion:

K. pneumonia isolates are increasing in Kerbala, with the blaIMP , blaVIM , and blaNDM being the most common carbapenemase genes. These strains are an emerging threat.

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