DOI: 10.4103/mjbl.mjbl_348_24 ISSN: 1812-156X
Molecular Detection of blaCTX-MG1 and blaCTX-MG2 Resistance Genes Among Uropathogenic Escherichia coli Isolates and Their Correlation with Antibiotic Resistance in AL-Hilla City
Salah Hasan Dukhna Al Quraishi, Hussein Oleiwi Mutaleb Al-Dahmoshi Abstract
Escherichia coli
(
E. coli
) is the most common cause for urine tract infections (UTIs) in 80% of patients from various groups. This study aimed to assess the uropathogenic
Escherichia coli
(UPEC) specimen obtained from midstream urine samples and their antibiotic resistance genes. A total of 327 specimens of midstream urine were collected. Phenotypically confirmed
E. coli
isolates were cultured on MacConkey agar and then on EMB agar, and its antibiotic resistance was tested by disk diffusion and then genotypically tested by PCR using marked genes of blaCTX-M. A total of 95 phenotypic
E. coli
isolates produced blaCTX-M, and the results for main groups blaCTX-M-gp-I and blaCTX-M-gp-II were 95 (100%) and 31 (32.63%), respectively. blaCTX-M-1 had 78 (82.1%) isolates, blaCTX-M-15, 92 (96.84%), and blaCTX-M-2, 28 (29.47%). The frequency of resistant genes blaCTX-MI was high in the isolated UPECs, (especially blaCTX-M 15). Isolates having multiple blaCTX-M genes constitute 87.36%.