Modulation of faecal microbiota of weaned piglets fed with a complete diet with Lupinus albus seeds

Abstract

In light of global sustainability challenges and the limited availability of conventional protein sources for livestock, it became essential to find alternative protein sources. In this context, the reintroduction of grain legumes into crop production systems may represent a promising strategy. Therefore, the aim of this study was to evaluate the replacement of soybean meal by Lupinus albus, on gut microbiota modulation in weaned piglets’ diet. Twenty weaned piglets (Landrace x Large White) were involved in the trial for 28 days. Animals were divided into two experimental groups balanced per weight and sex: the control (CTRL, n = 10) receiving basal diet with 7.3 % soybean meal and the treatment (LUP, n = 10) receiving 10 % of Lupinus albus ( L. albus ) meal. Microbiota analysis showed a limited modulation in terms of richness and evenness in LUP group compared to CTRL group. However, the dietary inclusion of lupin seed meal resulted in changes in the relative abundance of several bacterial genera, with higher abundances of Methanobrevibacter , a hydrogenotrophic methanogen associated with microbial fermentation, as well as Allobaculum and Romboutsia , which have been associated with the utilization of fibre and complex carbohydrates. In conclusion, the inclusion of L. albus as a replacement for soybean meal, in piglets’ diets could represent a viable strategy to reduce dependence on soybean protein by supporting local protein production systems, particularly suitable for organic farming as well as for extensive livestock systems supplying local markets.