DOI: 10.4103/mjbl.mjbl_485_24 ISSN: 1812-156X

Modify the Impacts of Oxidative Stress through Catalase Gene Polymorphism (CAT −89A>T) in Automotive Technicians

Shaimaa A. Al-Oubaidy, Mazin Eidan Hadi, Wael Rasheed Obaead Alfatlawi
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Abstract

Background:

One of the vital mechanisms used by cells to mitigate oxidative damage is the synthesis of antioxidant enzymes, such as butyrylcholinesterase, glutathione peroxidase, superoxide dismutase, catalase (CAT), and thioredoxin–peroxiredoxin.

Objectives:

This study was conducted to estimate the modification of the impacts of oxidative stress through the CAT gene polymorphism (CAT −89A>T) in automotive technicians.

Materials and Methods:

By using the single-strand conformation polymorphism (SSCP) technique, the age of the study groups was 33.30 ± 11.1 and 23.12 ± 2.3 years for workers and the control group, respectively, and the duration of work was 7.7 ± 4.9 years.

Results:

The SSCP electrophoresis produced three types of haplotypes (2H, 3H, and 4H) with a significant association with automotive technicians ( χ 2 = 11.71, P = 0.002), the oxidative stress state represented by reactive oxygen species (ROS) and total antioxidant (TAO) concentration; nonsignificant difference between study groups in ROS ( t = 0.446, P = 0.657) and a significant TAO elevation in workers ( t = 3.93, P = 0.000). The ROS concentration in the study groups according to (CAT −89A>T) haplotypes showed no significant differences among haplotypes ( f = 0.502, P = 0.734). Significant association of haplotypes and TAO concentrations; 2H and 4H have high level than other haplotypes ( P = 0.000).

Conclusion:

This study’s results concluded that significant association of (CAT −89A>T) with automotive technicians, a significant elevation in TAO in workers, and a significant association of promoter polymorphisms with TAO in workers.

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