MiPRIME: An integrated and intelligent platform for mining primer and probe sequences of microbial species
Zhiming Zhang, Jing Ren, Lili Ren, Lanying Zhang, Qubo Ai, Haixin Long, Yi Ren, Kun Yang, Huiying Feng, Sabrina Li, Xu LiAbstract
Motivation
Accurately detecting pathogenic microorganisms requires effective primers and probe designs. Literature-derived primers are a valuable resource as they have been tested and proven effective in previous research. However, manually mining primers from published texts is time-consuming and limited in species scop.
Results
To address these challenges, we have developed MiPRIME, a real-time Microbial Primer Mining platform for primer/probe sequences extraction of pathogenic microorganisms with three highlights: i) Comprehensive integration. Covering more than 40 million articles and 548,942 organisms, the platform enables high-frequency microbial gene discovery from a global perspective, facilitating user-defined primer design and advancing microbial research. ii) Employing a BioBERT-based text mining model with 98.02% accuracy, greatly reducing information processing time. iii) using a primer ranking score, PRscore, for intelligent recommendation of species-specific primers. Overall, MiPRIME is a practical tool for primer mining in the pan-microbial field, saving time and cost of trial-and-error experiments.
Availability
The web is available at {{https://www.ai-bt.com}}.
Supplementary information
Supplementary data are available at Bioinformatics online.