Metabolomic and structural signatures of pigmented and non‐pigmented
Himalayan
rice landraces
Sonal Aggarwal, Arun Kumar, Mohar Singh, Narpinder Singh Abstract
BACKGROUND
This study investigated the anti‐oxidant properties, starch composition, pasting behavior, structural properties, textural properties and non‐targeted metabolomic profiles of pigmented and non‐pigmented rice landraces as potential next‐generation functional food ingredients.
RESULTS
Pigmented rice demonstrated 1.34 times more anti‐oxidant activity as compared to non‐pigmented rice. Pigmented landraces showcased superior nutritional and functional attributes, including higher total dietary fiber and starch content. Fourier‐transform infrared (FTIR) analysis revealed distinct molecular signatures with enhanced peak transmittance, while X‐ray diffraction (XRD) indicated greater crystallinity ranging from 36–44.3% in pigmented rice compared with 30–40% in non‐pigmented rice, suggesting improved digestibility and processing versatility. Pigmented rice recorded less amylose content hence tended to possess increased adhesiveness values whereas non‐pigmented rice revealed greater amylose content hence was coupled with greater hardness values. Field‐emission scanning electron microscopy (FE‐SEM) images revealed that pigmented rice had densely packed and polygonal starch granules whereas non‐pigmented rice had loosely packed starch granules with intergranular voids. Untargeted gas chromatography–mass spectrometry (GC–MS) profiling identified 84 metabolites, including unique compounds such as 3,3‐dimethylbutanol and ethanoic acid, along with shared metabolites such as sucrose and linoleic acid, highlighting notable biochemical diversity. Multivariate statistical analyses using principal component analysis (PCA) and partial least squares‐discriminant analysis (PLS‐DA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway mapping further differentiated the metabolomic landscapes, with variable importance in the projection (VIP) scores identifying key bioactive contributors.
CONCLUSION
Pigmented rice landraces exhibited significant functional and nutritional advantages, making them promising candidates for functional food development and nutritional improvement programs. These findings support their potential role in advancing sustainable and health‐oriented food systems. © 2026 Society of Chemical Industry.