DOI: 10.1093/bioinformatics/btag422 ISSN: 1367-4811

Meta2DB: Curated shotgun metagenomic feature sets and metadata for health state prediction

Car Reen Kok, Nisha J Mulakken, James B Thissen, Jose Manuel Martí, Ryan Lee, Jacob B Trainer, Andre R Goncalves, Hiranmayi Ranganathan, Aram Avila-Herrera, Crystal J Jaing, Nicholas A Be

Abstract

Summary

Meta2DB is a curated metagenomic and metadata database that provides structurally consistent microbiome taxonomy feature count tables for 13,897 samples across 84 studies, 23 disease states, and 34 geographical locations. All samples were uniformly processed using a streamlined metagenomic classification pipeline that employs a unique and comprehensive reference database indexed to contain all sequences across all kingdoms of life that were present in the NCBI Nucleotide (nt) database retrieved on January 04, 2023. This pipeline leverages high-performance computing (HPC) resources at Lawrence Livermore National Laboratory and was used to process 50TB of publicly available raw metagenomic sequence data. Extensive metadata curation was carried out through a combination of manual curation and automated parsing, producing a consistent inter-study metadata table specifically structured to facilitate training of ML models for prediction of human health.

Availability

Data is available at https://gdo-meta2db.llnl.gov/ and https://zenodo.org/records/17315984.

Supplementary information

Supplementary data are available at Bioinformatics online.

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