Mechanism and cellular function of direct membrane binding by the ESCRT and ERES-associated Ca ²⁺ -sensor ALG-2

Apoptosis linked Gene-2 (ALG-2) is a multifunctional intracellular Ca ²⁺ sensor and the archetypal member of the penta-EF hand protein family. ALG-2 functions in the repair of damage to both the plasma and lysosome membranes and in COPII-dependent budding at

ndoplasmic eticulum xit ites (ERES). In the presence of Ca ²⁺ , ALG-2 binds to ESCRT-I and ALIX in membrane repair and to SEC31A at ERES. ALG-2 also binds directly to acidic membranes in the presence of Ca ²⁺ by a combination of electrostatic and hydrophobic interactions. By combining giant unilamellar vesicle-based experiments and molecular dynamics simulations, we show that charge-reversed mutants of ALG-2 at these locations disrupt membrane recruitment. ALG-2 membrane binding mutants have reduced or abrogated ERES localization in response to Thapsigargin-induced Ca ²⁺ release but still localize to lysosomes following lysosomal Ca ²⁺ release. In vitro reconstitution shows that the ALG-2 membrane-binding defect can be rescued by binding to ESCRT-I. These data thus reveal the nature of direct Ca ²⁺ -dependent membrane binding and its interplay with Ca ²⁺ -dependent protein binding in the cellular functions of ALG-2.