Luteococcus struthionis sp. nov. and Lacrimispora struthionigena sp. nov. isolated from ostrich faeces

Two mesophilic, Gram-stain-positive bacteria, OSA5 ^T and AGF001 ^T , were isolated anoxically from ostrich faecal material. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences revealed that strains OSA5 ^T and AGF001 ^T formed distinct lineages within the genera Luteococcus and Lacrimispora , respectively. Strain OSA5 ^T showed the closest relatedness to Luteococcus japonicus DSM 10546 ^T and Luteococcus sediminum DSMZ 27277 ^T with 96.6% 16S rRNA gene sequence similarity. Strain AGF001 ^T was closely related to Lacrimispora aerotolerans DSM 5434 ^T , with 97.4% 16S rRNA gene sequence similarity. Subsequent genomic analyses using overall genomic-based relatedness indices revealed that OSA5 ^T and AGF001 ^T shared average nucleotide identity and digital DNA–DNA hybridization values <95.0% and <70.0% with their close relatives, below the currently accepted thresholds for species-level delineation. The genomes of strains OSA5 ^T and AGF001 ^T were 3.2 Mbp and 5.4 Mbp, with G+C contents of 67.8 and 41.6 mol%, respectively. Based on phenotypic and genotypic data, strains OSA5 ^T and AGF001 ^T represent novel species in the genera Luteococcus and Lacrimispora , respectively; the names Luteococcus struthionis sp. nov. and Lacrimispora struthionigena sp. nov. are proposed. The type strains are OSA5 ^T (=CCM 9469 ^T =CCUG 78312 ^T =CECT 31229 ^T =DSM 119679 ^T ) and AGF001 ^T (=CCM 9472 ^T =CCUG 78314 ^T =CECT 31227 ^T =DSM 119664 ^T ), respectively.