Level of blood Tau and p‐Tau : a non‐invasive approach for the diagnosis of Alzheimer’s Disease(AD)
Sakshi Kumari, Yudhishthir Yadav, Abhinay Kumar Singh, Priyajit Kaur, Avinash Chakrawarty, Sharmistha Dey- Psychiatry and Mental health
- Cellular and Molecular Neuroscience
- Geriatrics and Gerontology
- Neurology (clinical)
- Developmental Neuroscience
- Health Policy
- Epidemiology
Abstract
Background
Prominent expression of Tau and phosphorylated Tau in cerebrospinal fluid (CSF) is one of the well‐known hallmarks of the Alzheimer’s disease (AD). High levels of p‐Tau can easily differentiate AD from other neurodegenerative disease. However, the serum levels of these proteins in AD patients are not well explored. This study provides an alternative non‐invasive approach to diagnose the disease by evaluating the level of Tau and p‐Tau181 in serum samples.
Method
Blood samples were collected from 39 AD patients, 37 MCI patients and 37 elderly individuals as controls. Quantification of tau and pTau in serum samples was done with the help of a label free real time Surface Plasmon Resonance technology, and further validated by Western Blot. Statistical analysis, including Receiver Operating Characteristic (ROC), was done for further affirmation.
Result
The concentrations of serum Tau and p‐Tau181 were significantly higher (p<0.00001) in AD (Tau; 47.49±9.00ng/µL, p‐Tau181; 0.161±0.04 ng/µL) compared to MCI (Tau; 39.26±7.78 ng/µL, p‐Tau181; 0.135±0.02 ng/µL) and were further compared to elderly controls (Tau; 34.92±6.58 ng/µL, p‐Tau181; 0.122±0.01 ng/ µL). A significant (p<0.0001) downhill correlation was found between Tau as well as p‐Tau181 levels with HMSE and MoCA score.
Conclusion
This study for the first time reports the concentration of Tau and p‐Tau181 in serum of AD and MCI patients. The cut‐off values of Tau and p‐Tau181 of AD and MCI patients with sensitivity and specificity reveal that serum level of these proteins can be used as a predictive marker for AD and MCI.