DOI: 10.1039/d5ra01044f ISSN: 2046-2069
LC-MS Orbitrap-based metabolomics using a novel hybrid zwitterionic hydrophilic interaction liquid chromatography and rigorous metabolite identification reveals doxorubicin-induced metabolic perturbations in breast cancer cells
Salah Abdelrazig, Áine McCabe, Alia Yasin, Rajneil Chaudhary, Michael A. Ochsenkühn, David Scicchitano, Shady A. AminThe Z-HILIC column enhanced LC-MS separation, sensitivity, selectivity, metabolite coverage, and stability compared to ZIC-pHILIC. The workflow enabled improved profiling of Dox-induced metabolic alterations in TNBC cells.