DOI: 10.4103/mjbl.mjbl_1319_23 ISSN: 1812-156X
Lactobacillus plantarum Gene Expression of Some Toxin Genes in Staphylococcus aureus
Rasha Zaid Tariq Ahmed, Rana Mujahid Abdullah Abstract
Background:
Staphylococcus aureus
bacteria is one of the most pathogenic bacteria that can infect the community and infections acquired in hospitals because of their ability to transmit infection, possession of toxins, virulence factors, and multiple resistances to antibiotics.
Objectives:
The study aims to evaluate the effect of
Lactobacillus plantarum
on the gene expression of some toxin genes in
S. aureus
.
Materials and Methods:
Two hundred and twenty bacterial samples were collected from various clinical sources and subjected to culture diagnosis, microscopic examination, biochemical tests and diagnosis with the Vitek device. The bacterium
L. plantarum
was obtained ready isolated from a dairy from the College of Engineering Agricultural Sciences, University and RNA was extracted and used for gene expression by quantitative PCR, is a well-established method for detecting and quantifying specific nucleic acid sequences.
Results:
After laboratory tests, 50
S. aureus
bacterial isolates were obtained from different clinical cases, and the highest isolation rate was in men by 54% and women by 46%. The results of the study show that the effect of
L. plantarum
supernatant bacteria was a non-significant increase in the gene expression of the
LukS/F
, whereas the study showed a significant increase in the genes (
eta
,
etd
) of
S. aureus
. The results showed the effect of
L. pantarum
decrease in gene expression of genes (
LukD/E, tst
), where the results of the
LukD/E
gene that were treated with
L. plantarum
recorded non-significant gene expression. Finally,
tst
gene recorded decreased significant differences in gene expression when treating with
L. plantarum
supernatant.
Conclusions:
L. plantarum
has proven effective in inhibiting some
LukD/E
and
tst
toxin genes for
S. aureus
bacteria, and
L. plantarum
can be used in the treatment of pathogenic bacteria and antibiotic resistance.