Outline
DOI: 10.1039/d6ay01589a ISSN: 1759-9660
Label-free fluorescent detection of miRNA-21 via EXPAR-triggered rolling circle amplification coupled with a dimeric/tandem G-quadruplex–thioflavin T reporter
Hanbo Zhang, Yuehua Yuan, Yulin Ren, Yubo Zhao, Yinxiao Qi, Feixue FengA sequential EXPAR–RCA cascade converts miRNA-21 recognition into tandem G-rich products, while a sequence-engineered tandem G4 reporter enhances ThT fluorescence for low-femtomolar detection.
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