DOI: 10.4103/mjbl.mjbl_1768_23 ISSN: 1812-156X

Isolation of Pseudomonas aeruginosa for Production of a Remarkable Antioxidant, Antibiofilm Molecule Pyocyanin

Fatima Mohammed Abd Al-Mohsen, Shaimaa Jassim Alsultany, Yazi Abdullah Jassim
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Abstract

Background:

Pseudomonas aeruginosa , which was distinguished and isolated from burn and wound injuries, produced a biomolecule or macromolecular polymer called pyocyanin, which has antioxidant and antibiofim capabilities against food pathogens.

Objectives:

This study aimed to determine how effective pyocyanin, which was extracted from P. aeruginosa , was as an antibiofim and antioxidant.

Materials and Methods:

An average of 40 P. aeruginosa specimens were collected from various sources. Including burns, wounds, and urinary tract infections (UTI), from Hilla hospitals, from October 2022 to April 2023, the isolates were diagnosed as P. aeruginosa by culturing on selective media, biochemical tests, and confirmed diagnostic by Vitek-2. In this investigation, different bacterial species were isolated from pathological samples, Escherichia coli , Klebsiella pneumoniae , Staphylococcus aureus , and Enterococcus faecalis . Pyocyanin was extracted and characterized, then the 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) assay was used to assess pyocyanin’s ability to scavenge free radicals. Using 96-well microtiter plates, the antibiofim impact of pyocyanin against different pathogens was assessed.

Results:

Biochemical tests and microscopic diagnosis showed agreement in all isolates with characteristics of the chosen bacterial species. While the Vitek 2 device was used to confirm the findings for isolating organisms up to 99%. The results demonstrated the ability of pyocyanin to scavenge DPPH free radicals, which were revealed by observing the color change in the microtiter plate from DPPH’s original purple to yellow. Pyocyanin’s ability to reduce DPPH became more active as its concentration increased. Pyocyanin expressed antibiofilm activity by increasing its concentration reach to 100% in S. aureus , also 100% for E. faecalis , as the pyocyanin concentration increases, the absorbance will decrease.

Conclusion:

From this study, we conclude the ability of P. aeruginosa to produce pyocyanin, as well as proving its antioxidant and antibiofilm activities.

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