DOI: 10.11648/j.cbe.20251002.12 ISSN: 2578-8884

Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating Faba Bean (<i>Vicia faba. </i>L.)

Andualem Turito, Kamel Argaw, Dereba Workineh
Show PDF Cite
Natural process of fixing molecular nitrogen to ammonium is called biological nitrogen fixation. By supplying nitrogen to legume plants, it helps to support sustainable agriculture globally. The most significant leguminous crop, faba beans, can coexist symbiotically with soil bacteria called rhizobia. The purpose of this study was to isolate, describe, and assess Rhizobium nodulating faba bean's symbiotic efficacy. YEMA-CR was used to isolate a total of 24 Rhizobium isolates. Every isolate was described using YEMA based on how they responded to phenotypic traits. Every isolate tested positive for BTB, catalase, and indole and was found to be motile, gram-negative, rod-shaped, and incapable of absorbing Congo red. The pattern of intrinsic antibiotic resistance revealed that whereas the majority of isolates were sensitive to tetracycline, the majority were tolerant to ampicillin and pencillin. Every isolate exhibited nodulation in sand culture. All examined metrics showed significant (<i>p<0.05</i>) improvements in plants, according to analyses of variance. Sixty-six percent of isolates were deemed highly successful based on symbiotic effectiveness. According to the results, the study region included Rhizobia bacteria that might be utilized as bio-fertilizer. Therefore, in order to better classify Rhizobium isolates and develop them for inoculants, more research on their field conditions and molecular characterization is required.

More from our Archive