iPSC‐derived cortical neurons of sporadic Alzheimer’s disease patients reflect their clinical vulnerability
Féodora Bertherat, Bryan Ng, Nora Bengoa‐Vergniory, Becky C. Carlyle, Richard Wade‐Martins- Psychiatry and Mental health
- Cellular and Molecular Neuroscience
- Geriatrics and Gerontology
- Neurology (clinical)
- Developmental Neuroscience
- Health Policy
- Epidemiology
Abstract
Background
iPSC‐derived cortical neurons from sporadic Alzheimer’s Disease (AD) patients were used to compare cellulare phenotypes in response to AB or tau insults with clinical data of the individual patients.
Method
We generated14 iPSC lines from sAD patients from the Deep and Frequent Phenotyping study, which has collected a battery of clinical data from early‐stage sAD patients. Phenotypes such as neurite loss, axonal branching or cytotoxicity were measured in iPSC‐derived cortical neurons following Amyloid Beta oligomer treatment.
Result
Individual AD patient lines exhibit significantly different levels of neurite loss and decreased axonal branching due to Amyloid Beta. This differences is significantly correlated with clinical vulnerability of the individual patients.
Conclusion
Differences in disease vulnerability in patients observed in clinic is reflected in patient iPSC lines when treated with Amyloid Beta.