DOI: 10.4103/mjbl.mjbl_282_23 ISSN: 1812-156X
Investigation of some virulence factors in Staphylococcus aureus
Fatima Salem Obaid Al Jasmi, Ali Abdul Kadhim Al-Ghanimi Abstracts
Background:
Staphylococcus aureus
bacteria produce a wide range of virulence factors that increase the virulence and pathology of these bacteria, the most important of which are the enzyme hemolysin, leukocidin, and protease.
Objectives:
This study amid to investigate some virulence factors of
S. aureus
isolates from different clinical sources.
Materials and Methods:
Twenty-four
S. aureus
were collected from the beginning of December 2021 to March 2022 from different clinical samples from the reviewers of the hospitals of the Holy Kerbala Governorate, followed by cultivation and diagnosis by microscopic and molecular examinations. Some virulence genes were investigated, such as the gene encoding the beta-hemolytic enzyme, as well as the leukocidin toxin, and then the hemolysin and protease enzymes were investigated by conventional methods.
Results:
Twenty-four isolates of
S. aureus
were obtained by diagnosing them by microscopy and molecular examination using
femA
. The activity of protease and hemolysin was also tested traditionally in the isolates, as well as the investigation of the leukocidin
lukED
and hemolysin
hlb
gene.
Conclusion:
In general, we note from the results obtained from this study that a high percentage of
S. aureus
bacteria contain virulence factors genes represented in the
hlb
and
lukED
genes as well as protease enzyme and decrease in the activity of the hemolysin enzyme in the tested isolates.