Invasive and Non-invasive Biomarkers to Study the Genetic Instability among Polycystic Ovarian Syndrome Women of South Indian Cohort
Anirban Goutam Mukherjee, V. G. Abilash, L. Hariprasath, S. P. Tamilarasi, S. Nishu
A
BSTRACT
Background:
Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic abnormalities. Emerging evidence suggests that genomic instability may contribute to its pathophysiology and long-term health consequences.
Aim:
The aim of this study was to evaluate genomic instability in women with PCOS using invasive and non-invasive cytogenetic biomarkers.
Settings and Design:
A case–control study was conducted among women with PCOS and age-matched healthy controls from a South Indian cohort.
Materials and Methods:
Buccal epithelial cells and peripheral blood samples were collected from 200 women with PCOS and 100 healthy controls. Genomic instability was assessed using the buccal micronucleus cytome (BMCyt) assay, cytokinesis-block micronucleus (CBMN) assay and chromosomal aberration analysis.
Statistical Analysis Used:
Data were analysed using GraphPad Prism 6.0. Group comparisons were performed using Student’s unpaired
Results:
Women with PCOS exhibited increased frequencies of nucleoplasmic bridges, nuclear buds, micronucleated binucleated cells and chromosomal aberrations compared with controls. Higher frequencies of chromosomal breaks, chromatid breaks and dicentric chromosomes were also observed, indicating increased genomic instability in the PCOS group.
Conclusion:
Women with PCOS demonstrated increased cytogenetic abnormalities suggestive of elevated genomic instability. The combined application of BMCyt, CBMN and chromosomal aberration analyses provides complementary information regarding deoxyribonucleic acid damage and chromosomal instability in PCOS and may be useful for genomic instability assessment in future studies.