Intriguing Biological Perspectives of Actinomycetes from Two Various Commodities
S. Mary Essolin, R. S. DhivyaThe actinomycetes from two different marine commodities were isolated, screened, and characterised in the current investigation. Twelve distinct bacterial isolates were obtained and tested using morphological, microscopic, cultural, and biochemical characterisation techniques. The selected bacterial isolates exhibited strong antagonistic activity against pathogens and food spoilage microorganisms. The Kirby–Bauer disc diffusion method was used to measure the antagonistic properties of the crude extracts. Isolate AMWM showed good antagonistic activity against the test organisms in comparison with the other isolates and was selected for further study. 16S rRNA gene sequencing was used to identify the active strains, and a phylogenetic tree was constructed. The 16S rRNA gene sequence of isolate AMWM showed complete homology with Streptomyces lincolnensis. The isolated bioactive metabolites were structurally characterised by FTIR and HPTLC.