DOI: 10.38053/acmj.1960743 ISSN: 2718-0115

Intermittent fasting modulates autophagy-related gene and protein expression in the aged rat liver

Aysun İnan Genç, Ruken Ceylani
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Aims: This study aimed to investigate the effects of a 35-day intermittent fasting (IF) regimen on the transcriptional and protein expression profiles of key autophagy-related markers in the liver of aged male Wistar rats. Methods: Twelve-month-old male Wistar rats were assigned to either an IF group or a control group. The IF group was subjected to an 18:6 h time-restricted feeding protocol for 35 days, whereas control animals had ad libitum access to food and water. Hepatic mRNA expression levels of ATG5, ATG7, Beclin1, LC3, and p62 were quantified by quantitative real-time PCR. In addition, the protein expression of these autophagy-related markers was evaluated by immunohistochemistry. Results: IF significantly increased the hepatic mRNA expression of ATG5, ATG7, Beclin1, and LC3 (p

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