DOI: 10.1128/spectrum.01695-26 ISSN: 2165-0497
Integrated genomic and phenotypic analysis of intra-clade variation in
Candida auris
from a New York City tertiary hospital
Shivani Satia, Jennifer Pfeiffer, Abdullah Kilic, E. Yoko Furuya, Marie C. Smithgall, Mahesh Mansukhani, Susan Hsiao, Jun Shi, Rinki Kumar, Daniel A. Green, Gregory J. Berry, Fann Wu ABSTRACT
Candida (Candidozyma) auris
(
C. auris
) poses an urgent public health threat due to its widespread emergence, antimicrobial resistance, and ability to cause severe infections and outbreaks associated with high mortality rates. In this study, we phenotypically and genotypically characterized antifungal susceptibility profiles and used whole-genome sequencing (WGS) to assess overall genetic diversity and to identify clonal clusters. From July 2020 to December 2025, a total of 118
C. auris
isolates from 97 patients were collected at a tertiary hospital in New York City. Antifungal susceptibility testing and WGS were performed on all clinical isolates to characterize their clade-specific antifungal resistance genes and intra-clade genetic variations. Ninety-nine percentage of the
C. auris
isolates demonstrated phenotypic fluconazole resistance. Resistance to echinocandins was observed in 7.6% (9/118) of isolates, and resistance to amphotericin B was found in 54% (64/118). WGS confirmed that all 118 isolates belonged to the CDC-defined Clade Ic. Azole resistance mutations in the
ERG11
and
CDR1
genes were detected in 100% (118/118) of the isolates, and mutations in the
TAC1B
gene were present in 92.4% (109/118) of the isolates, including one case with a unique set of mutations (
ERG11
-Y132F,
CDR1
-E709D,
TAC1B
-A583S). Mutations in the
FKS1
gene were identified in 5.9% (7/118) of isolates though these were not consistently linked to echinocandin resistance. Genomic analysis also revealed that individual patients frequently carried the same strain throughout the study. Furthermore, multiple strains emerged simultaneously across the hospital each year. This suggests that numerous patients were likely already colonized upon admission, rather than acquiring the pathogen through intra-hospital transmission. The presence of multiple
C. auris
strains with varying resistance patterns poses significant therapeutic challenges. Genomic typing can help hospitals understand the diversity of their
C. auris
isolates to track their spread, identify potential outbreaks, and ensure the effectiveness of infection control measures.
IMPORTANCE
Candida (Candidozyma) auris
is an emerging, multidrug-resistant fungal pathogen designated by the Centers for Disease Control and Prevention (CDC) as an urgent public threat. Traditionally,
C. auris
infections have been considered predominantly nosocomial, with outbreaks typically attributed to a single clonal lineage spreading within healthcare facilities. However, the genomic analysis performed in our study identified multiple distinct clusters of genetic variations within a single clade, suggesting that infections were not derived from a single source. This genomic diversity indicates that many patients may have acquired
C. auris
prior to hospital admission. Nevertheless, intra-hospital transmission was evident, as patient-to-patient spread facilitated the dissemination of various clonal lineages within the healthcare environment. These findings underscore the complex epidemiology of
C. auris
and highlight the urgent need for stringent infection control measures both within and beyond hospital settings.