DOI: 10.1111/jmi.13250 ISSN: 0022-2720

Influence of different surface cleaning methods on STED‐FCS and scanning STED‐FCS calibration measurements

Agnes Koerfer, Francesco Reina, Christian Eggeling
  • Histology
  • Pathology and Forensic Medicine
Show PDF Cite

Abstract

Molecular mobility is an important measure in biological functionality, as molecules have to diffuse to meet and interact and perform actions. Measurement of mobility requires specific tools such as fluorescence correlation spectroscopy (FCS). Especially, combination with super‐resolution stimulated emission depletion microscopy (STED‐FCS), whether in a point‐ or beam‐scanning mode, has proven valuable for determination of anomalous diffusion. STED‐FCS however relies on an accurate calibration of the effective observation spot formed for different laser powers of the additional STED laser. This poster article highlights the need for calibration measurements and outlines that rather simple procedures involving acetone cover‐glass surface cleaning only, instead of Piranha cover‐glass surface cleaning, and point instead of more complex scanning STED‐FCS are sufficient for calibration.

This article is protected by copyright. All rights reserved

More from our Archive