In vitro
                    <scp>NAD</scp>
                    + supplementation increases
                    <scp>CD4</scp>
                    + and
                    <scp>CD8</scp>
                    + T cell function in people with

doi:10.1111/hiv.70342

DOI: 10.1111/hiv.70342 ISSN: 1464-2662

In vitro NAD + supplementation increases CD4 + and CD8 + T cell function in people with

Guangming Li, Salma Sharaf, Yaoxian Lou, Lishan Su, Shyam Kottilil, Poonam Mathur

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Abstract

Objectives

HIV perturbs T cell homeostasis and disrupts immune function, increasing the risk of age‐associated comorbidities. Ageing people with HIV (PWH) have exacerbated expression of CD38 on T cells, which depletes intracellular nicotinamide adenine dinucleotide (NAD+). We investigated whether boosting intracellular NAD+ could restore T cell homeostasis and function.

Methods

We collected blood samples from virally suppressed PWH to determine the effects of in vitro NAD+ on CD38 expression and T cell function in response to anti‐CD3/CD28 antibodies stimulation in PBMCs culture supplemented with IL‐2 and IL‐7.

Results

We observed that NAD+ supplementation not only reduced expression of CD38 but also improved T cell function with an alleviated exhaustion phenotype in response to anti‐CD3/CD28 antibodies stimulation.

Conclusions

If confirmed in clinical trials, NAD+ supplementation has the potential to restore T cell homeostasis and immune function in PWH and ameliorate age‐associated comorbidities.