DOI: 10.4103/mjbl.mjbl_50_23 ISSN: 1812-156X
In-Vitro Activity, Extraction, of Bioactive Agents Produced by Streptomyces Spp. against Multidrug-Resistant Pseudomonas aeruginosa Isolated from Clinical Samples
Zainab Hasan Abed, Eman Mohammad Jarallah Abstract
Background:
An antibiotic was discovered in
Streptomyces
spp. during secondary screening of the
Actinomycetes
-producing antibiotics program.
Objectives:
The aim of the article was to extract and isolate a powerful antibiotic made by
Streptomyces
that was efficacious against strains that were multidrug resistant. Additionally, the antibiotic was examined using Fourier transform-infrared spectrum, gas chromatography–mass spectrometry, and ultraviolet–visible spectroscopy.
Materials and Methods:
Yeast malt extract agar medium (International
Streptomyces
project ISP2) with 1 mg/mL nystatin as an antifungal drug is the most selective medium for
Actinomycetes
growth.
Result:
Streptomyces
spp. and
Pseudomonas aeruginosa
were identified by specific-species 16SrRNA primer. These
Streptomyces
isolates were screened for test; the antibacterial activity by cross-streaking method against 30 clinical isolates of
P. aeruginosa
that showed resistance to 17 antibiotic types
Streptomyces
isolates with potential antibacterial activity were mass cultivated, and ethyl acetate was used to extract secondary metabolites. Among the
Streptomyces
spp. isolated strains, four isolates (st4, st6, st11, and st12) demonstrated significant antibacterial activity with inhibition zone ranging from 0 to 22 mm against
P. aeruginosa
.
Conclusion:
The four
Streptomyces
spp. isolates (st4, st6, st11, and st12) showed higher antimicrobial metabolites activity; so these extract isolates were elected for characterization by several methods.