Impact of Photoactivated Curcumin on Proliferation of Experimentally Induced Periodontitis and Diabetic Gingival Fibroblast Cell Line
Ananya Sharma, Vivek Kumar Bains, Chetan Chandra, Ruchi Srivastava, Sunakshi Soi, Utkarsh Singh, Aditya Bhushan PantABSTRACT
Objective:
To evaluate the impact of photoactivated curcumin on proliferation of experimentally induced periodontitis and diabetic gingival fibroblast in laboratory settings.
Materials and Methods:
Gingival fibroblast (GF) cells were divided into healthy GF (HGF), diabetic GF (DGF), and periodontitis-associated diabetic GF (P-DGF) cells that were treated with a solution of curcumin that was prepared and diluted in autoclave distilled water to obtain a concentration used (1 mg/ml). Gingival fibroblasts with curcumin and without curcumin were seeded in a 96-well plate that was treated with a light-emitting diode curing light with a wavelength of 445 nm using a transparent diffuser tip. All healthy (HGF) as well as diseased (DGF and P-DGF) gingival fibroblasts were thus treated with curcumin (c-only), photoactivation (p-only), and both photoactivated curcumin (pc-both) and were then histologically analyzed for evaluation of cell proliferation and viability of fibroblasts. The number of proliferated cells/proliferative values were calculated by relative fluorescence values displayed by a fluorimeter. The number of viable cells (%viability) correlates with the magnitude of dye reduction and expressed as percentage of Alamar-Blue reduction.
Results:
HGF group treated with p-alone, c-alone, and pc-both showed a significant increase in the fluorescence value and proliferation in the cells at 24 to 48 and 48 to 72 h, whereas for the DGF group, there was a statistically significant increase in the fluorescence value and proliferation when treated with p-alone from 24 to 72 h, with a significant decrease in the proliferation of the cells when treated with c-alone and pc-both at 24 to 48 h and 48 to 72 h. For the P-DGF group, a significant decrease in the fluorescence value and proliferation was observed when the cells were treated with p-alone and c-alone at 24 to 72 h.
Conclusion:
Photoactivation (p-alone) was the most efficient in depicting a highly significant increase in the percentage viability for the group P-DGF and DGF at 24 h, whereas a significant increase in the fluorescence value and proliferation was observed for the group P-DGF when treated with photoactivated curcumin (pc-both) from 24 to 72 h.