Identification of the consistently differential expressed hub mRNAs and proteins in lung adenocarcinoma and contraction of the prognostic signature: a multidimensional analysis
Yiran Liu, Zhenyu Li, Qianyao Meng, Anhui Ning, Shenxuan Zhou, Siqi Li, Xiaobo Tao, Yutong Wu, Qiong Chen, Tian Tian, Lei Zhang, Jiahua Cui, Liping Mao, Minjie Chu- General Medicine
- Surgery
Background:
This study aimed to elucidate the consistency of differentially expressed hub mRNAs and proteins in lung adenocarcinoma (LUAD) across populations and to construct a comprehensive LUAD prognostic signature.
Methods:
The transcriptomic and proteomics data from different populations were standardized and analyzed using the same criteria to identify the consistently differential expressed mRNAs and proteins across genders and races. We then integrated prognosis-related mRNAs with clinical, pathological and EGFR mutation data to construct a survival model, subsequently validating it across populations. Through plasma proteomics, plasma proteins that consistently differential expressed with LUAD tissues were screened and validated, with their associations discerned by measuring expressions in tumor tissues and tumor vascular normalization.
Results:
The consistency rate of differentially expressed mRNAs and proteins was approximately 20%- 40%, with ethnic factors leading to about 40% consistency of differential expressed mRNA or protein across populations. The survival model based on the identified 8 hub mRNAs as well as stage, smoking status, and EGFR mutations demonstrated good prognostic prediction capabilities in both Western and East Asian populations, with a higher number of unfavorable variables indicating poorer LUAD prognosis. Notably, GPI expression in tumor tissues was inversely correlated with vascular normalization and positively correlated with plasma GPI expression.
Conclusion:
Our study underscores the significance of integrating transcriptomics and proteomics data, emphasizing the need to account for genetic diversity among ethnic groups. The developed survival model may offer a holistic perspective on LUAD progression, enhancing prognosis and therapeutic strategies.