Identification and Determination of the Major Constituents in Xie-Bai-San by Ultra-performance Liquid Chromatography–Mass Spectrometry
Bao-Xin Li, Peng-Yue Li, Li-Xia Hao, Xiao-Xia Xue, Sha-Sha Kong, Qin-He Zhao, Yun-Tao DaiObjective:
The ancient classical Chinese formulation, Xie-Bai-San (XBS), was studied qualitatively and quantitatively, and its main components were characterized.
Materials and Methods:
The chemical compounds in XBS were identified using a combination of ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry and molecular networking (MN) analysis. In addition, a multiple reaction monitoring (MRM) quantification method was established using ultra-high-performance liquid chromatography–triple quadrupole mass spectrometry. Mulberroside A, kukoamine B, liquiritin, and glycyrrhizic acid were quantitatively analyzed in ten batches of XBS using this method.
Results:
Using clusters of MN and databases, 91 compounds were rapidly identified, including stilbene glycosides, saponins, flavonoids, and alkaloids. Of these, 30, 33, and 28 compounds were from
Conclusions:
This study provides a comprehensive and reliable method for the identification and quantitative analysis of XBS.