ID #717 Cerebrospinal fluid circulating tumor DNA as marker of measurable residual disease for CNS germ cell tumors – an international pediatric brain tumor liquid biopsy collaborative
Yoshiko Nakano, Robert Siddaway, Mansuba Rana, Richard Yuditskiy, Cyril Li, Ian Burns, Andrew Bondoc, Immanuela Okeke, Nali Huang, Yanlai Tang, Guoping Shen, Libin Huang, Arun Kumaran Anguraj Vadivel, Anthony Arnoldo, Jamie Thurber, Thomas Dodsworth, Evelyn Ruoyun Lu, Dennis Tak-Loi Ku, Matthew Ming-Kong Shing, Peiyi Yang, Mei Jin, Chantel Cacciotti, Shayna Zelcer, Samuele Renzi, Xiaoling Zhang, Juliette Hukin, Enrica Tan, Yen-Lin Liu, Bo Li, Birgit Ertl-Wagner, Peter Dirks, James Rutka, Adrian Levine, Derek Tsang, Anirban Das, Julie Bennett, Vijay Ramaswamy, Liana Nobre, Ute Bartels, Eric Bouffet, Annie Huang, Uri Tabori, Cynthia Hawkins, Anthony Pak-Yin LiuAbstract
Background
Liquid biopsy enables profiling of CNS tumors without surgery and tracking minimal residual disease. Our pilot work indicated high detection rate of circulating tumor DNA (ctDNA) from CSF of patients with CNS germ cell tumors (CNS-GCT) at diagnosis. Herein, we present an expanded, international dataset with longitudinal sampling to evaluate the dynamics of CSF-ctDNA.
Methods
A multi-institutional cohort of CNS-GCT patients treated with induction chemotherapy and response-adapted radiation was assembled. CSF was collected at diagnosis, during therapy, and after completion of treatment. Cell-free DNA was extracted for studying copy-number variations (CNVs) using low-pass whole-genome sequencing, with additional mutational analysis by panel sequencing when available. ctDNA positivity is inferred by the presence of CNVs +/- mutations.
Results
One-hundred and fifty-eight samples from 77 patients (median age 13 years; germinoma=48, NGGCT=29) were analyzed. At diagnosis, ctDNA was detected in 95% (37/39) of germinoma, and 88% (15/17) of NGGCT patients, including all patients with negative tumor markers (n = 21). Where mutational analysis was performed (n = 27), oncogenic drivers in the form of amplifications or mutations were identified in 96% (26/27). Longitudinal CSF profiling revealed persistent ctDNA in 27% of samples (6/22) after 1-2 cycles of chemotherapy, and in 25% (6/24) after induction therapy, prior to irradiation. Among patients with persistent ctDNA post-induction, three developed early recurrence despite negative ctDNA following radiation, and one demonstrated refractory disease, representing only events to-date. Evolution of CNV profiles was observed from paired-CSF at diagnosis and relapse, suggesting emergence of resistant clones.
Conclusion
Liquid biopsy enables the minimally invasive diagnosis and genotyping of CNS-GCTs. Early clearance of ctDNA was observed in the majority, while persistence following induction therapy may serve as a poor prognostic marker. Evidence based on the largest entity-specific CSF cohort supports the prospective incorporation of serial CSF liquid biopsies for personalized, response-adapted, management of CNS-GCTs.