Hydnocarpic acid and chaulmoogric acid, the principal antifungal compounds in
Hydnocarpus hainanensi
s
seed extract, inhibit f
Yanqiu Zhu, Hong Guo, Xiaozhou Xu, Wenqiang Gao, Daowan Lai, Linlu Qi, Jinlan He, Qitong Lin, Xiaoying Zhu, Qingyu Wang, Zixue Wu, Deng Chen, Jiyao Feng, Sen Pang, Vijai Bhadauria, Wensheng Zhao, You‐Liang Peng, Zhigang Li, Zhenhua Zhang, Jun Yang Abstract
BACKGROUND
Environmentally sustainable management of plant fungal diseases requires green agrochemicals that combine high efficacy with novel modes‐of‐action. Rice blast caused by Magnaporthe oryzae is a destructive crop fungal disease, and developing botanical fungicides with new antifungal mechanisms is urgently needed for its green control.
RESULTS
In this study, we report that an ethanol‐petroleum ether crude extract of Hydnocarpus hainanensi s seeds (designated DF2) strongly suppresses infection of the rice blast fungus M. oryzae . Bioactivity‐guided fractionation of DF2 identified hydnocarpic acid and chaulmoogric acid as the principal antifungal constituents, which inhibit conidial germination, appressorium formation and plant infection by M. oryzae . Furthermore, treatment with DF2 significantly perturbed carnitine‐related metabolites and severely attenuated fatty acid β‐oxidation in M. oryzae conidia. Interestingly, exogenous addition of methylenecyclopropyl acetic acid to the M. oryzae conidial inoculum mimicked these effects, whereas supplementation with
CONCLUSION
Taken together, our findings indicate that hydnocarpic acid and chaulmoogric acid disrupt fatty acid β‐oxidation and impede infection of M. oryzae , and suggest that DF2 could serve as a potential botanical fungicide to control fungal diseases of crops. © 2026 Society of Chemical Industry.