Genomic profiles of anatomically distinct single-lobe and multi-lobe gliomas
Ying Wang, Zhansheng Fang, Kang Xie, Jingxing Leng, Kang Yang, Tao Song, Tiangxiang Huang, Jun Wu, Longbo ZhangBackground:
A critical aspect of glioma biology is their presentation as either single-lobe gliomas (SLGs) or multi-lobe gliomas (MLGs). Although MLGs are often presumed to represent more advanced disease stages, emerging evidence suggests that they may arise as distinct biological entities with unique genetic and molecular characteristics.
Methods:
In this multi-center retrospective study, we conducted a comprehensive genomic and molecular analysis of 174 glioma samples collected from 2018 to 2021, focusing on the mutational landscapes associated with SLGs and MLGs. Tumor samples were collected during operation and stored at –80 ° C. All DNA sequencing was performed using Illumina HiSeq. Tumor sites were defined based on pre-operative magnetic resonance imaging scans. R package “Maftools” was used for visualization of genomic mutations and statistical analyses.
Materials and results:
Our findings revealed that MLGs are associated with significantly shorter progression-free survival compared to SLGs. While canonical glioma-associated mutations, such as those in
Conclusion:
This study delineates the genetic and molecular distinctions between SLGs and MLGs, offering new insights into glioma heterogeneity and disease progression. These findings highlight the need to account for anatomical dispersion when interpreting glioma genomic profiles and could inform future stratified treatment approaches.