DOI: 10.1039/d6sc06723a ISSN: 2041-6520
Genetic code expansion for site-specific encoding of a switchable, intrinsic fluorophore–quencher pair to monitor protein dynamics
Priyanda Giri, Yarra Venkatesh, Moriah H. Mathis, Christina M. Hurley, Chloe M. Jones, Olivier Ndogo Eteme, Zachary M. Hostetler, Richard B. Cooley, Rahul M. Kohli, Ryan A. Mehl, E. James PeterssonAcridonylalanine (Acd) and methyltetrazinyl phenylalanine (Tet) form a genetically encodable fluorophore–quencher pair for which quenching can be switched off by biorthogonal or photochemical reactions of Tet.